Reliability of Cobas Amplicor PCR test in detection of Mycobacterium tuberculosis in respiratory and nonorespiratory specimens
نویسندگان
چکیده
منابع مشابه
Detection of Mycobacterium tuberculosis complex in sputum specimens by the automated Roche Cobas Amplicor Mycobacterium Tuberculosis Test.
Three hundred twenty-four sputum specimens from 151 patients with suspected active pulmonary tuberculosis were tested for the presence of the Mycobacterium tuberculosis complex with auramine fluorochrome stain and automated PCR assay (Roche Cobas Amplicor Mycobacterium Tuberculosis Test [MTB]). The results were compared with those of the conventional Löwenstein-Jensen tube culture and the BACTE...
متن کاملEvaluation of Cobas Amplicor MTB test to detect Mycobacterium tuberculosis in pulmonary and extrapulmonary specimens.
Tuberculosis (TB) is one of the major public health problems in the world. Effective control of TB depends on rapid and correct diagnosis and appropriate treatment. The aim of this study was to evaluate the performance of Cobas Amplicor MTB (CA-MTB) test for pulmonary and extrapulmonary specimens isolated in our laboratory. A total of 424 specimens obtained from the suspected TB patients from J...
متن کاملComparison of the COBAS AMPLICOR MTB and BDProbeTec ET assays for detection of Mycobacterium tuberculosis in respiratory specimens.
The performances of the BDProbeTec ET (Becton Dickinson) and COBAS AMPLICOR MTB (Roche) were retrospectively evaluated for detecting Mycobacterium tuberculosis complex in various respiratory specimens. The BACTEC and MGIT liquid culture system (Becton Dickinson) was used as a reference method. A total of 824 respiratory specimens, comprised of sputa, bronchoalveolar lavage fluid, and bronchial ...
متن کاملComparison of the automated Cobas Amplicor Mycobacterium tuberculosis assay with the conventional methods for direct detection of Mycobacterium tuberculosis complex in respiratory and extrapulmonary specimens.
OBJECTIVE To evaluate the recently available Cobas Amplicor polymerase chain reaction (PCR) system for the detection of Mycobacterium tuberculosis complex (MTBC) in well-characterized clinical specimens and to compare the results with clinical classification, conventional culture and staining techniques. METHODS Three hundred and forty-four clinical specimens consecutively received for cultur...
متن کاملComparison of In-house PCR with Conventional Techniques and Cobas Amplicor M. tuberculosis™ Kit for Detection of Mycobacterium tuberculosis
PURPOSE Polymerase chain reaction (PCR) assay, introduced as a fast and sensitive diagnostic method, is useful in detecting Mycobacterium tuberculosis. The purpose of this study was to evaluate the usefulness of in-house PCR assay in the detection of Mycobacterium tuberculosis by comparing PCR results with conventional diagnostic techniques and Cobas Amplicor M. tuberculosis kit. MATERIALS AN...
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ژورنال
عنوان ژورنال: Military Medical and Pharmaceutical Journal of Serbia
سال: 2009
ISSN: 0042-8450,2406-0720
DOI: 10.2298/vsp0912992l